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Cell line development

Discuss your cell line development project with usBPS Bioscience offers specialist cell line development services, including knockout or overexpressing cell lines, reporter cell lines, co-stimulatory cell lines, CAR-T cells and bioluminescent cells. Engineered by expert scientists, these cell lines are tailor-made for applications including, protein production, pathway analysis, inhibitor/activator studies, cellular controls for antibody staining, CAR-T modelling, and high-throughput screening systems.

With a wealth of custom resources, BPS Bioscience’s cell lines are custom-designed to accelerate research objectives meeting your exact specifications, ensuring high quality for every project.

Benefits of using this cell line development service

  • Cell lines developed by specialists
  • Service includes knockout or overexpressing cell lines, reporter cell lines, co-stimulatory cell lines, CAR-T cells & bioluminescent cells
  • Custom-designed to meet your exact specifications
     

Overexpression cell lines
  • High throughput screening tool to identify receptor/pathway inhibitors
  • >25 available promoters & >30 parental cell lines
  • Customise with your gene promoter of interest
  • Luciferase, eGFP or custom reporter systems
Overexpression cell lines
  • Positive control for antibody or ligand screening
  • Target for CAR-T, BiTE or antibody killing
  • Study protein expression effects on cell signaling, proliferation & metabolism
  • Varying expression levels (high, medium, low) to mimic natural variations

Overexpression cell lines
  • CRISPR/Cas9 genome editing ensures stable knockout condition
  • Knockout of immune-mediators such as TCR, B2M & CIITA for universal CAR-T cells
  • Study protein KO effects on cell signaling, proliferation, metabolism & activation

Overexpression cell lines
  • For use in co-culture with reporter cells
  • High-throughput inhibitor screening
  • TCR activator & customised expression of co-stimulators or checkpoint inhibitors

Overexpression cell lines
  • Expression of your CAR on immune-derived or other cell lines to model CAR-T function in co-culture studies
  • Cell lines or primary CD4+/CD8+ T cells

Custom cell line development process

  1. Molecular biology: expression vectors generated
  2. Selection & pool generation: parental cells transfected/virally transduced & cell pool is selected using antibiotics and phenotypical characterisation
  3. Limiting dilution & clonal selection: based on initial pool testing, single cell-derived clones selected
  4. Confirmation of expression: analysed by Western blot or flow cytometry
  5. Functional validation: cells treated with reference control compound for dose-response if applicable. Cells may be assayed in co-culture, depending on objectives
  6. Stability testing: desired number of clones selected for passage stability testing. Mycoplasma testing & cell banking services also available

Applications
• Protein production
• Pathway analysis
• Inhibitor/activator studies
• Cellular controls for antibody staining
• CAR-T modelling
• High-throughput screening systems

Materials available for download
BPS Bioscience cell systems services brochure

How can we help you?

If you're ready to get started, contact our cell line development specialists to discuss your project with us.

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