Mobilised human CD34+ progenitor cells

We have partnered with ZenBio to provide mobilised peripheral blood CD34+ cells for research purposes.

CD34+ progenitor cells are derived from healthy donors that have undergone G-CSF mobilisation to stimulate the bone marrow, which produces a large number of multipotent haematopoietic and progenitor stem cells, and mobilises them into the peripheral blood stream. CD34+ progenitor cells are in a phenotypically undifferentiated state and contain two main cellular sub-populations; haematopoietic and endothelial CD34+ progenitor cells. Once isolated, these freshly prepared CD34+ progenitor cells are immediately cryopreserved.

CD34+ progenitor cells can be used in studies involving directed differentiation into committed types of blood cells and endothelial lineages.

Benefits of using these CD34+ progenitor cells
• Lot QC tested for surface marker characterisation
• Immediately cryopreserved after isolation
• High post thaw viability
• Guaranteed purity of 95%
• Viability of >80%
• Serology screening of cells
• Samples fully consented including for genetic analysis and commercial research purposes

CD34+ progenitor cell pathogen testing
All cryopreserved CD34+ cells are tested negative for HIV-1, HIV-2, HTLV I, HTLV II, Syphilis, CMV, HBV and HCV via PCR. However, no known test can offer complete assurance that the cells are pathogen free. Products are tested and are free from mycoplasma contamination. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.

To prevent clumping and to maximise viability upon thawing, we strongly suggest the use of our Lymphocyte Medium (LYMPH1, LYMPH-1-50) to thaw these cells, especially if you are using a homemade formulation and not achieving success.

To request a quote or find out more, please contact our ZenBio specialist.

These products are for use in Biomedical Research only. They are not to be used for any form of human treatment, or for any purpose that is prohibited by law, including any use for human reproductive cloning. It is your responsibility to ensure that you have necessary licensing and ethics approvals in place for the use and storage of any human tissue related material supplied by Cambridge Bioscience.